Today I am going to walk you through the easiest way to grow psilocybe cubensis (stay away from the penis envy variety though, as it grows poorly with this method) at home. Please note that this method will NOT work for cylocybe tampanesis, panaeolus cyanescens, cylocybe galindoi etc...
We will not be growing in manure with this method. We will be using brown rice flour, vermiculite and water, placing the subtrate into glass jars, sterilizing, inoculating with cubensis spores, allowing the mycelium to colonize the substrate, then ultimately fruiting these brown rice flour cakes.
***Let me start by saying cleanliness and sterile technique are the most important part of mycology. This will make or break you, so take the necessary steps to do it right or your failure rate will skyrocket. You have been warned.***
Supplies needed for substrate and mixing:
- 1/2 pint mason jars with lids and rings (or two piece lids if that makes more sense to you). You need wide mouth jars. The ones that taper toward the top will not work.
- Brown rice flour. My local grocery stores don't carry it, but Whole Foods, Sprouts, or other health food stores that cater to weak people like those sensitive to gluten should carry it. If younsee very pale looking people who look vegan, ask where they shop, amd youll find it there. This is what is common around here:
- tap water or bottled water, but not distilled water
- Vermiculite. This can be found at home depot, lowes, gardening stores. You do not want the coarse grain if you have options. The smaller grain size is better. (No need for a huge bag)
- 1 Large mixing bowl
- 1 fork for stirring and breaking everything up (I like using larger forks for this)
- 1 spoon for putting substrate into jars once mixed
- 1 Large pot with lid (a pressure cooker is the correct way of doing this, and the only way to know you are 100% sterile, but a pot with a lid works just fine unless you decide to really take this seriously)
Prep and supplies for sterile technique:
- disposable powder free gloves. I like exam gloves.
- 90% rubbing alcohol.
- purell hand gel
- 1 lighter (a Bic will work just fine)
- Spray can of lysol
- Antibacterial dish soap
- Work space with good light, flat counter space, and room to spread supplies out a bit (I use my kitchen)
- roll of tinfoil
Supplies for growing and prep:
- Drill and 1/4" drill bit, and 1/8" bit (can substitute this smaller bit with a nail or punch)
- 60-70 quart clear plastic storage tote with lid. I like the ones with the two latches on top because they allow for a better seal. You want the lid to be clear as well not just the tote.
- Bag of perlite (need enough to cover bottom 4 inches of the tote, so no need to go huge here)
- Hygrometer (humidity and temperature gauge)
And of course you will need viable cubensis spores. There are many sources online for these. They are sold for research purposes only, so do not mention what you will be doing with the spores in your order, or communication with these companies. There are certain states that do not allow these types of spores to be imported and that will be stated on all the websites. Some simply state it and will ship irrelevant, but others will not. Find a reputable source for spores, as most are horrible (nothing worse than getting a contaminated syringe from a vendor who gives no shits about their product or customers). If you need a reputable source, send me a PM. Remember that all cubensis, in theory, have the same potency, except penis envy (which you shouldn't grow with this technique). "A cube (cubensis) is a cube, unless it's penis envy." Some cubes are larger mushrooms, some are smaller, some yield better than others etc... but potency really boils down to your environment, unless you isolate and clone (I'm not getting into agar on petri dishes, nor liquid cultures on this thread - just keeping it simple).
Supplies needed for after harvest:
- food dehydrator
- scale with decent accuracy (a digital scale will work just fine from ebay. You just need to make sure it measures small units. Dosing the finished product should never be a guessing game, since you can never un-take mushrooms, aND a half gram too much for certain people can be WAY too much, instead of the perfect experience. Don't rely on your visual judgement of quantity, use a scale.)
Instructions:
1. Ok, let's get right into it. You're going to either drill or punch holes in the lids to the mason jars with something small (1/8th"). These will be the inoculation holes as well as air holes, so they just need to be big enough to get the needle of the syringe through without scraping the sides. Make sure the lid is on a flat surface when you do this so you dont bend it, especially if using a punch or nail. There will be 4-5 holes total, so one at 12:00, one at 3:00, one at 6:00, one at 9:00, then if you're doing the 5th hole, dead center (not necessary, but encouraged). You are shooting for something like this:
2. Clean your mason jars, lids and rings with Antibacterial dish soap and hot water, then set aside to dry. I like to let them air dry overnight, upside down on a rack. If you let them dry rightside up, they can catch contaminates. Don't dry with a dish towel, as that towel is not clean, even if you just took it out of the washer and dryer.
3. Once the jars and lids are dry, grab your big mixing bowl, brown rice flour, vermiculite and measuring cup. You will be mixing vermiculite to brown rice flour to water in a 2:1:1 ratio. To make enough for approximately 12 1/2 pint jars you will use 6 cups vermiculite, 3 cups brown rice flour, and 3 cups of water. Dump in vermiculite and brown rice flour first. Stir them up so they get mixed well. Now pour in the water. I like to use warm water for this since it seems to mix easier. Don't dump in all the water at once or the mixing process will get all fucked up. Add half the water, mix well with a fork, then add .75 cup, mix well, then add in the last .75 cup and mix. Each time you add water, you arent dumping it in, but pouring slowly and spreading it out around the brf and vermiculite mixture. You are going for a consistency of wet sand, no lumps. Each stage of mixing you will want to break up the chunks and lumps with the fork. This is not a quick process to get it to the correct consistency. Once it reaches field capacity it should look similar to this:
4. Grab your jars and spoon, then scoop the brf mixture in the jars one by one, filling to about 85-90% capacity (where the ring gets stopped when you screw it on is a good gauge). You can shake the jars side to side if you have large gaps in the substrate, but absolutely don't pack it. When mycelium starts to grow it needs air. Small cracks/gaps in the substrate are no big deal, and in most cases help the mycelium grow. Let everything be nice and loose. Don't fucking pack it!
5. Once jars are loaded to 85-90%, take a paper towel and clean off the inside of the jar above the substrate, plus the rim. You are trying to clean off any bits of substrate that touched anywhere above top of the substrate line. Use different parts of the paper towel when you do this, as you arent trying to smear it, you are trying to clean! Clean well since this is a great spot for bacteria to grow, which in turn will contaminate your jar.
6. Now take the dry vermeculite and top off the jar almost all the way to the top (leave small gap between top and where the lid will be), with a clean spoon (not the brf scooping spoon unless you wash it first), then smooth off the top so it's flat. This will act as a filter to catch contaminates, but also provide for air exchange (again, mycelium needs air). Don't pack this shit either, assholes! From this point on, do not turn the jars at an angle. Keep them upright. Any contaminates that land on the filter of vermiculite will be caught and stay in place. If you tilt the jar, the vermiculite shifts and moves, and as a result the bad shit gets moved to your substrate, then you're in fucksville. Remember this any time you touch your jars, shitdicks!
7. Put lids and rings on. You want a good seal, so screw on well. Don't over tighten though, fuckasses.
8. You will be covering the tops of each jar with tinfoil squares. When measuring the tinfoil, it needs to cover the lids, plus some of the jar. The tinfoil will be acting as a barrier so steam from the pot doesn't get into the holes of the jars. If steam gets into the jars, it will fuck up the field capacity of the substrate. Standard roles of tinfoil are 12" wide, so a simple way to measure is just split down the middle so it's 6" wide, then measure 6" up and walla a 6"x6" square. Set the square on top of the lid so it's centered, then apply pressure to top, sides of ring, jar groove under ring, and then the jar itself. Your working from top to bottom tightening and molding the tinfoil so everything is nice and tight. The most important area will be the base of the ring, because if steam or boiling water gets under the tinfoil, this will be the best and last barrier to keep it from getting to the lid, so make sure that's especially tight. Don't just press the area below the ring, kind of twist while you press as well. Some use rubber bands in this area, but if you do it right, they are not necessary.
9. Take your pot and fill with about an inch and a half of water. The jars cannot touch the bottom where the heat source is, so either put a rack in the bottom (if using a rack, use less water and watch your boil since you don't want it to boil up and get under the tinfoil), or go ghetto style and take your trusty tinfoil and pull out a long roll of it and shape it into a snake, then coil it in the bottom, starting at the edges and working towards the center (ghetto style removes the threat of boiling water getting under the tinfoil for the most part, so I would recommend this, even if you have a rack). Now set your jars on top of it, but make sure they are secure/not going to be jolted from boiling water. Remember, we are not boiling the jars, we are steaming the jars. If you have a nice and deep pot, jars can be stacked, just make sure they are offset from the lower ones. If you do not have a deep pot, you may have to do this in two separate batches, depending on how wide the pot is. Cover, bring water to a boil, then turn heat down so it's still bubbling/boiling, but not at a rapid rate. Start your timer now for 90 - 120 minutes. Once time is up, turn off heat, move pot off the burner, and let cool naturally - with the lid still on. Do not help it cool. This is a long waiting process, so doing it at night, then going to sleep while it cools is nice.
10. Your jars and substrate are now considered sterilized (unless you have a pressure cooker, in which case they would be absolutely sterilized, aND in less time). Leave them in the pot with the lid on until you are ready to inoculate with spores. You want to make sure the center of the substrate isn't still hot, so I'd say a minimum of 8 hours (I usually wait longer, but I've heard of people getting away with 6 hours). If the center of the substrate's temperature, which you can't feel by holding the jar, is too hot, you will kill the spores. Better to play it safe, imo.
11. Ok, now here is where we need to step up the sterile technique. Close any Windows in the house, shut off fans, AC, heater, etc... You do not want any moving air in your workspace, as germs are everywhere and that can and will contaminate everything. Now clean your workspace. Start with spraying the air with lysol in the immediate and surrounding area. Wait a few minutes. Now wipe down actual workspace with lysol.
12. Move the following to the workspace: spore syringe, paper towels, exam gloves, alcohol, face mask, lighter, purell, substrate jars.
13. Scrub hands and arms up to elbows with Antibacterial soap, dry with paper towels. Put on exam gloves, grab purell and squirt some on your gloved hands. Rub the purell all over (All fucking over, you lazy fucks) the gloves and wait for it to dry.
14. Grab alcohol, paper towels, syringe, and lighter. Put a decent amount of alcohol on a paper towel and wipe down the lighter. Do the same to the syringe. Now shake the fuck out of the syringe. You are trying to separate the spores in the spore solution (they seem to join together in clumps when left alone, so you want to shake the solution until the spores are as spread out as possible), so by shaking vigorously for about a minute straight, you accomplish this pretty well. Some syringes need more shaking than this to break everything up (if you still see a clump, or multilevel clumps, keep shaking). Play it by ear based on what you can see. Some syringes have no visible spores, but that doesn't mean shit. Shake the fuck out of either. Keep in mind spores are not visible to the naked eye when in solution, unless they join together. Any given spore syringe has countless spores, even if you don't see shit.
15. Grab the jars out of the pot. Put on face mask (your breath and your dirty whore mouth have bacteria). Remove tinfoil, discard tinfoil. Pat lids and jars dry with paper towels (steam will have come up from the substrate, hit the tinfoil cover, then rested back on the lid, so pat dry). Dry tops first, then work your way down. Do not use the same paper towel for the next jar, as the lids are the areas that will be most sensitive to bacteria and contaminents. Take alcohol and paper towels to clean the lids of each jar, making sure you aren't using a used portion of the paper towel on the next jar lid (new dab, new section of paper towel). Don't worry about the ring, just the lid surface and around the holes.
16. Take needle out of packaging, remove plunger from syringe and screw needle onto syringe (leave protective cap on needle when you do this). Wipe down protective cap of needle with alcohol.
17. Flame sterilize the needle with your lighter. To do this you will be running your needle back and forth through the flame of the lighter. Use the blue part of the flame so you dont get the black burn residue on the needle. After you get the needle nice and hot, concentrate on the tip portion until it is glowing red. You're now ready to inoculate your first jar.
18. Insert the needle into the first hole of your jar, and angle it so it slides down the side of the glass so you can see it. Now with it pressed against the glass slowly press the plunger until just a drop of the spore solution comes out. Do this same thing to the rest of the holes in the jar.
19. When moving onto each additional jar, flame sterilize first and repeat the process. Flame sterilization is necessary only when moving to a new jar, not each separate hole of the same jar.
20. Now move the inoculated jars somewhere out of the way where they get decent ambient light, but not direct sunlight. A book shelf or something like that where they won't be bumped or disturbed. I like to put all my jars back in the box they came in for easy moving. Now you wait for mycelium growth which will take 5-14 days in most cases. When checking for growth do not tilt the jars at all. It's best to leave them on a flat surface and just spin them.
To be continued...